By P. Turner
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Extra resources for Animals in Scientific Research: An Effective Substitute for Man?: Proceedings of a Symposium held in April 1982 under the auspices of the Humane Research Trust
Toxicol. 2, 419-442. Dean, D. J. and Hodson-Walker, G. (1979). An in vitro chromosome assay using cultured rat liver cells. Mutation Res. 64, 329337. de Serres, F. J. and Ashby, J. (1981). Evaluation of Short-Term Tests for Carcinogens. Elsevier/North Holland, Amsterdam. Garner, R. , Miller, E. C. and Miller, J. A. (1972). A. 1530. Cancer Res. 32, 2058-2066. 43 Hanawalt, P. , Cooper, P. , Ganesan, A. K. and Smith, C. A. (1979). DNA repair in bacteria and mammalian cells. Ann. Rev. Biochem. 48, 783-836.
The mode of addition of the test substance is an important consideration in the use of an in vitro test as it is in vivo. A knowledge of the chemical and physical properties of the sample is very valuable in selecting the dosage form. The age, stability and purity of a sample must also be considered. One of the greatest stumbling blocks in the so-called blind trials has been the failure to take into account the importance of variations in physicochemical properties of test substances as a contributor to apparent inconsistencies in in vitro test results .
And Cooling, C. (1967) The respiration of human liver tissue. , 16, 721-724. Brown, D. , Langley, P. , Smith, D. and Taylor D. C. (1974) Metabolism of chloroform I. The metabolism of 14C-chloroform by different species. Xenobiotica, 4, 151-163. Burk, R. F. and Lane, J. M. (1979) Ethane production and liver necrosis in rats after administration of drugs and other chemicals. , 50,467-468. 25, Department of Health and Social Security, London, page 2. Elder, J. B. Gangoli, P. C. and Gillespie, I. E.